Batsman Gayle, one of the biggest drawcards in world cricket, has not represented West Indies since the 2015 World Cup, while Samuels has been absent for a year because of a dispute with the board.
Neither plays for a test side whose much-mourned decline in fortunes was much in evidence during the innings and 209-run defeat at the hands of England at Edgbaston last week.
Sunil Narine, Dwayne and Darren Bravo all made themselves unavailable for the five-match ODI series, which begins in Manchester on Sept. 19 and concludes in Southampton on Sept. 29.
"The panel welcomes back Chris Gayle and Marlon Samuels, who will both add value to our batting and help with the nurturing of the young batsmen in the team," CWI chief selector Courtney Browne said in a statement.
"Both Sunil Narine and Darren Bravo have declined to be considered for ODIs in England.
"Narine has however confirmed his desire to play 50-over cricket again but has asked to play in our Regional Super 50 before he is considered and Darren said he was not available for selection.
"Dwayne Bravo said he is still not 100 percent fit, but is looking at a possible return to international cricket next year."
Gayle, who turns 38 next month, has scored 9,221 runs in 269 ODIs for the Caribbean islands, while Samuels has scored 5,180 in 187.
The pair share the record for the highest partnership in one-day cricket with the 372 they combined for against Zimbabwe in Canberra during the last World Cup.
Samuels refused a retainer from the board last year and was dropped for deciding to play in the Pakistan Super League rather than the Caribbean's Super 50 competition.
Squad: Sunil Ambris, Devendra Bishoo, Miguel Cummins, Chris Gayle, Jason Holder (captain), Kyle Hope, Shai Hope, Alzarri Joseph, Evin Lewis, Jason Mohammed (vice captain), Ashley Nurse, Rovman Powell, Marlon Samuels, Jerome Taylor, Kesrick Williams.
chris gayleDarren BravoDwayne BravoEngland vs West Indies 2017England vs West Indies ODIsSunil Narine
First Published: August 22, 2017, 10:31 AM IST