The 29-year-old has enjoyed a successful start to his international career, taking 5-57 in his first Test innings against South Africa at The Oval in July.
"Middlesex Cricket are extremely disappointed to announce that the result of the medical scan which Toby undertook yesterday (Thursday) has revealed that he has a stress fracture in his L5 vertebra (lower back), which will mean that he will take no further part in the domestic season for Middlesex," said a statement.
Roland-Jones -- who spearheaded Middlesex's championship-winning campaign last season -- has taken 17 wickets in four Tests at an average of 19.64.
England will announce their Ashes squad on Wednesday, with the first Test starting in Brisbane on November 23.
First Published: September 22, 2017, 9:50 PM IST