The 39 pujas are winners of West Bengal's 'Biswa Bangla Sharad Samman' award.
The event, presided over by Chief Minister Mamata Banerjee, will provide a window of two to three minutes each to every Puja committee to showcase their endeavours in a tableau-like formation.
Around 10,000 visitors and 2,000 VIPs are expected to watch the floats from barricaded seating areas set up on either side of the road, the city police said.
Smaller puja committees are allowed to display a maximum of three tableaux while bigger ones can highlight as many as five floats.
Several arterial roads, including Mayo Road, have been closed to traffic for the three-hour event.
"Spectators coming by trams or buses will have to alight at Esplanade or Park Street and walk it down to the venue," the police said.
For ease of viewing, four giant screens have been placed at vantage points along the stretch.