Holder Petra Kvitova suffered a shock 3-6, 7-5, 6-4 defeat by Serbia's former world number one Jelena Jankovic in the Wimbledon third round on Saturday.
Second seed Kvitova had breezed through the first two rounds but after taking the first set against 30-year-old Jankovic she made too many errors allowing the 28th seed to triumph.
Twice champion Kvitova looked confident at the start, having dropped only three games in her first two matches, and when she broke Jankovic in the fourth game she looked set to progress.
The 25-year-old broke again in the third game of the second set on a sun-drenched Centre Court and went 4-2 ahead as Jankovic struggled to match her power around the court.
But the experienced Serb had battled through two three-set matches already at the All England Club and knew how to dig deep as she fought back to 4-4 before forcing a decider.
The final set went with serve until Jankovic, who had begun to find the shots that saw her reach the U.S. Open final and top the rankings in 2008, broke Kvitova to close out the match.