The world championship bronze medallist combo of Gutta and Ponnappa lost 18-21 12-21 to third seeded Korean pair of Ye Na Jang and So Young Kim in a 40-minute contest.
The 2011 World Championship bronze medallists made short work of New Zealand pair Anna Rankin and Madeleine Stapleton by defeating them 21-10, 21-7.
"We are the best and there is no doubt about it now and we remain the best for quite some time now," said Gutta.
Jwala Gutta and Ashwini Ponnappa crashed out of the Bitburger Open after suffering a straight-game defeat against Hui Ern Ng and Hui Lin Ng.
The Commonwealth Games gold-medallist pair brushed aside Germany's Anika Dorr and Annika Horbach 21-14 21-11 in a 23-minute match.
Jwala Gutta and Ashwini Ponnappa and a few other Indians will start their campaign in the Bitburger Open Grand Prix Gold badminton tournament which starts with the qualifiers at Saarbrucken, Germany.
With Commonwealth and Asian Games coming up next year, Ashwini Ponnappa and Jwala Gutta might be joining hands once again.
The two, after parting ways after the London Olympics last year, are likely to be seen in action as early as October.
Doubles shuttler Ponnappa will join hands with former partner Gutta once again, starting from the Denmark Open from October 15-20.
The two teams were locked 2-2 after four matches before Ponnappa and Fischer sealed it for Pune.
Saurabh Verma and the mixed doubles pair of Ashwini Ponnappa and Joachim Fischer Nielsen helped Pune Pistons to 4-2 win over Krrish Delhi Smashers in the IBL.
The top Indian shuttlers said the doubles event is not being taken seriously by the administrators of the sport and the focus is on singles.
The Commonwealth Games women's doubles gold medallists broke their successful partnership after the Olympics.
Sindhu lost her semi-final match against Ratchanok Intanon of Thailand, to settle for a bronze at World Badminton Championships.
The World No.3 will be leading the Indian challenge at the World Championships starting on Monday.
At the World Championship, there are no qualification rounds and only shuttlers inside the top 50 world rankings are eligible to participate.
Vimal said since Bangalore had already bought Kashyap as an icon player, the team had to give a miss to Ashwini.
Rupesh and Thomas told that like Jwala Gutta and Ashwini Ponnappa, their base price also was slashed without their knowledge.
The Indian Badminton League defended their decision to slash the base price of two of the six Icon Players, Ashwini Ponnappa and Jwala Gutta.
For the last couple of days, the Indian Badminton League has attracted a fair bit of attention. There has been a controversy as well but once the dust settles, focus will turn to what the league can accomplish. As Digvijay Singh deo reports, the signs are positive.
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