The Jamaican sprint star clocked a season's best of 19.78 seconds, easing up a full 40 metres from the line, to advance along with Canadian Andre De Grasse as the second automatic qualifier.
Social media users demand an apology from Ellen DeGeneres.
Bolt, who is desperate to better his 2009 world record best of 19.19sec, clocked 20.28sec in winning his first round heat, easing up a full 50 metres from the line.
Did boisterous celebrations over an Olympic victory by the world's fastest man lead to a false alarm about gunfire and a panicked evacuation of Kennedy Airport?
Guess only the fastest man on the planet can afford to do that!
After claiming the 100m gold on Sunday, Bolt said he was confident of winning the 200m and 4x100m relay this week, which will give him the treble of golds for three straight Olympics.
From Beijing brilliance to Rio Romp, the world has never seen a sprinter like the Jamaican thunder Usain Bolt,
The 29-year-old Jamaican legend, competing in his final Olympics, powered over the line in 9.81sec, vanquishing drug-tainted American rival Justin Gatlin who took silver with Andre De Grasse of Canada claiming bronze.
Usain Bolt booked his place in the final of the Olympic 100m final on Sunday, winning his semi-final in a time of 9.86 seconds.
Bolt won his 100metres heat after what he called a sluggish start, but looked pained after the race as he prepared to fend off the challenge of American Jason Gatlin for the Olympic crown.
Ten days of competition in Rio de Janeiro's Olympic Stadium get under way with officials keen to draw a line under a miserable 12 months which has left the athletics' image languishing at an all-time low.
Jamaican sprinter Usain Bolt breaks out into the samba dance and says he is looking to break more records at the 2016 Olympic Games.
Olympic champion Usain Bolt shook a tail feather with a group of samba dancers and showing off his trademark celebration step, "The Lightning Bolt", at a media event on Monday.
Usain Bolt, the most decorated sprinter of all time, is oozing with confidence as he edges closer to realizing his 'Triple-Triple' gold medal dream at the Olympic Games in Rio.
Usain Bolt has encouraged his fans to come and watch him make history for one last time at the ongoing Rio Olympics 2016.
That is a stark contrast with the London Games in 2012, when tickets for the most popular events were sold out several months in advance.
Usain Bolt will launch an audacious bid for three Olympic sprint gold medals for an unprecendented third time as the perfect antidote to a year of turmoil in his drug-tainted sport.
He will join the Jamaican pre-Olympic camp as he seeks to become the first man to win gold medals in the 100, 200 and 4x100 relay at three successive Games.
Gatlin had slammed Jamaica of giving preferential treatment to Bolt by picking him for the Rio Olympics despite the fact that he missed trials due to an injured hamstring.
President, PM, MPs to Take 30% Pay Cut for 1 Year as Covid-19 Infects Economy; MPLAD Funds Suspended
WHO Warns Masks ‘No Silver Bullet’, Slams Racist Calls for Africa to be Testing Ground for Vaccine
Shadow Boxing or Fight Against Covid-19? Amid Crisis, K'taka-Kerala Border Closure Sparks Debate
Coronavirus Can Last on Face Masks for a Week, Banknotes for Days: Study